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degU [2018-09-05 14:14:12]
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degU [2018-09-05 14:14:12]

two-component response regulator, regulation of degradative enzyme expression, [SW|genetic competence], [SW|biofilm formation], capsule biosynthesis (together with [protein|search|SwrAA]), non-phosphorylated DegU is required for swarming motility
locus
BSU35490
pI
5.60
mw
25.72 kDa
protein length
229 aa Sequence Blast
gene length
687 bp Sequence Blast
function
regulation of degradative enzymes, [SW|genetic competence], and other adaptive responses
product
two-component response regulator
essential
no
synonyms
sacU,iep
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    3,644,607 → 3,645,296

    Phenotypes of a mutant

  • defect in [SW|biofilm formation] [Pubmed|20815827], this can be suppressed by [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-independent expression of [protein|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|BslA] [Pubmed|21742882]
  • the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants due to reduced expression of the [gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE] operon [Pubmed|24296669]

    • The protein

    Catalyzed reaction/ biological activity

  • transcription activator and repressor (see [SW|DegU regulon])
  • DegU-P represses expression of the fla/che operon (''[gene|1C6C8DB02E10EAC0588389D9F0D3AF3E19172C5C|flgB]-[gene|CC423DE6481353699B0CD3A2B8BBC36967A832CC|flgC]-[gene|7E8F88F7A9CAD4ED0927CDC6AAFDEDC08197C709|fliE]-[gene|EB815705133E3318F655F6024B7D9BA587FD1DDA|fliF]-[gene|8DDCC3D139ACCB635BF014946E1282A72D535390|fliG]-[gene|4D8CF3BED8F94DE3F8FCDEB1FE55D5F1D17FE26F|fliH]-[gene|401459F105BC8A8342341D953AE259D6C3868AB6|fliI]-[gene|600DECF2BE9ADCAB87E53790CE4F0D61F8B3F7CC|fliJ]-[gene|E7D735A16C35FBC635CFDB92ECB1C84F442D0DF0|ylxF]-[gene|42277DE1030E6FEB416EAE381CD40A54D49736FF|fliK]-[gene|819FAE47ADC9A4FE55C8F2105E19FA7EE286FC0C|flgD]-[gene|DC906ED8D787602B5796BAD8FD81F02C4BAC8D13|flgE]-[gene|2CCB8AD9238D18BA2361020A671844C021E81EE0|fliL]-[gene|1D51AF3E6456F126203D7C7273FB828A47E26DFB|fliM]-[gene|2E73F8DD89F7CFCDE7FAFC58220D8171D0913BAC|fliY]-[gene|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]-[gene|C2C67880EDF09E1BA75A4791628EE1982F9B6B0B|fliZ]-[gene|6ED6C3DDC4F3142FD01D32840D955B7E4A19F385|fliP]-[gene|A3C07B70C87D671979D053A6272A0FCDED6F3BB7|fliQ]-[gene|9856F25F23264AD1402A85AE9E25F10B68CAD739|fliR]-[gene|68DB2871A88535714C84FE86006AB54CEB6F0EAD|flhB]-[gene|974FA844E263AA694477992FA50468CB8EFAB807|flhA]-[gene|BB6F5D7463EF96E2C6344D0BC30A227C5E3B5817|flhF]-[gene|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|flhG]-[gene|B0415A8CD040EC714B54F4038E065B3E1E20A271|cheB]-[gene|6B3B222E56BF0C95A2371CA5208B5522B44D4689|cheA]-[gene|887D84520DF3D5F22DED525C1C17130EDE60DC36|cheW]-[gene|DC9B5446484E42F46EB2B0541192D7D8B4B3AC8F|cheC]-[gene|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD]-[gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-[gene|347ACF1D4E2D0C1B4E9DF0DEA9807A6A7D1519AC|swrB]'') in the absence of [protein|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|SwrAA/2], and switches to an activator of the operon in the presence of [protein|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|SwrAA/2] [Pubmed|24386445]

    • Modification

  • phosphorylated by [protein|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|DegS] on Asp-56 [Pubmed|1901568], this modulates DNA-binding activity
  • phosphorylation by [protein|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|DegS] occurs in response to inhibition of flagellar rotation [Pubmed|28800172,23888912]

    • Effectors of protein activity

  • DNA-binding activity of DegU is inhibited by [protein|E1744329B6489F989F93F3E71E51E772E3926ABF|RapG] [Pubmed|12950930]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P is degraded by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [Pubmed|20070525]

    • Structure

  • [PDB|4GVP] (VraR from ''Staphylococcus aureus'', 35% identity) [Pubmed|23650349]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P is degraded by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [Pubmed|20070525]
  • accumulation of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P results in decreased transcription of the [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO] and [gene|8C13E1EF5437DD8F9906643DEDCBB18DABE3B9E3|tapA]-[gene|09B082BF39703F0D9A5980E643175DBD59F5B228|sipW]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA] operons due to increased levels of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]-P [Pubmed|24123822]
  • information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000030 PRODORIC2 database]

    • Expression and Regulation

    Operons

    genes
    [gene|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS]-[gene|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]
    description
    [Pubmed|1688843]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1688843], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|18502860], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|23123903], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|24317403], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

    • regulation

  • induced by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|23123903]
    • view in new tab

    genes
    [gene|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]
    description
    [Pubmed|18502860]

    regulation

  • induced by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|23123903]
    • view in new tab

    Biological materials

    Mutant

  • BKE35490 (Δ[gene|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
  • BKK35490 (Δ[gene|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 20030732,19118340,23927648,24988880

    • The [SW|DegU regulon]

  • 12471443

    • Original Publications

  • 17850253,8878039,14563871,1901568,1321152,1459944,18194340,18978066,10908654,18502860,10094672,19389763,20815827,21742882,18414485,19420703,2428811,7746142,18502860,19389763,19416356,19734658,1688843,20070525,18197985,15598897,12950930,17590234,8955341,22496484,22745669,23123903,21965392,24123822,24296669,122328658,23888912,24149708,24317403,25431404,25433860,24386445,25777015,25880922,25887289,25755103,26819068, 23650349,27026185,27920766,28800172,29124898